This work aimed to evaluate the skin-beneficial properties of <i>Agastache rugosa</i> Kuntze, an natural medicine used to deal with several types of problems in conventional people medication. The whole phenolic compounds and complete antiradical, nitrite scavenging, superoxide scavenging, antielastase, and antihyaluronidase actions of a scorching water extract of <i>A. rugosa</i> Kuntze leaves (ARE) have been spectrophotometrically decided. Intracellular reactive oxygen species (ROS) was fluorometrically quantitated utilizing 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA). Inducible nitric oxide synthase (iNOS) and filaggrin have been evaluated utilizing Western evaluation. Actual-time quantitative RT-PCR was used to measure filaggrin mRNA. <em>Caspase</em>-<em>14</em> exercise was decided utilizing a fluorogenic substrate.
ARE contained the entire phenolic content material of 38.9 mg gallic acid equal/g extract and exhibited 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide radical, and nitrite scavenging actions with the SC<sub>50</sub> values of two.9, 1.4, and 1.7 mg/mL, respectively. ARE exerted suppressive actions on nitric oxide (NO) and ROS ranges elevated by lipopolysaccharide (LPS) or tumor necrosis factor-<i>α</i> (TNF-<i>α</i>) in HaCaT keratinocytes. It attenuated the LPS-stimulated expression of iNOS. ARE augmented the UV-B-reduced filaggrin expression on each protein and mRNA ranges and was able to upregulating the UV-B-reduced <em>caspase</em>-<em>14</em> exercise. ARE inhibited <i>in vitro</i> elastase and hyaluronidase actions related to the wrinkling course of. ARE, on the concentrations used, didn’t intrude with the viability of HaCaT keratinocytes.
These findings preliminarily indicate that the leaves of <i>A. rugosa</i> possess fascinating beauty potentials, comparable to anti-inflammatory, barrier protecting, and antiwrinkle actions, which infers their pores and skin therapeutic potentials. After 11 days of testosterone alternative within the OGT group, SC rats have been submitted to a saline injection, whereas SG, OG and OGT rats obtained glycerol. All rats have been euthanized three days after injections. OG rats introduced larger serum creatinine and urea, and sodium excretion, in comparison with SC and SG, whereas testosterone attenuated these modifications. Acute tubular necrosis was additionally mitigated by testosterone. Renal immunostaining for macrophages, lymphocytes and NF-κB was larger in OG in comparison with SC and SG. As well as, renal interleukin-1β, <em>Caspase</em> Three and AT1 gene expression was larger in OG rats in comparison with SG.
Astragalus polysaccharide has a protecting impact on hematopoiesis in an irradiated mouse mannequin and reduces apoptosis in megakaryocytes
Huangqi, the dried root of Radix Astragali, is a vital herb in Conventional Chinese language Drugs and has been used to advertise hematopoiesis for hundreds of years. Astragalus polysaccharide (ASPS), the bioactive compound of Huangqi, serves an important position in hematopoiesis. The purpose of the current examine was to research the hematopoietic results, specifically the thrombopoietic results, and the molecular mechanisms of ASPS utilizing an irradiation‑induced myelosuppressive mouse mannequin. Colony‑forming unit assays, stream cytometric evaluation of apoptosis, ELISAs, Giemsa staining and western blotting have been carried out to find out the hematopoietic and anti‑apoptotic results of ASPS.
The outcomes demonstrated that ASPS enhanced the restoration of purple blood cells at day 21 following therapy, in addition to platelets and white blood cells at day <em>14</em>. As well as, ASPS promoted colony formation in all lineages (megakaryocytes, granulocyte monocytes, erythroid cells and fibroblasts). The morphological examine of the bone marrow demonstrated that tri‑lineage hematopoiesis was preserved within the ASPS‑ and thrombopoietin (TPO)‑handled teams in contrast with the management group. The general cellularity (imply complete cell depend/space) of the ASPS‑handled group was just like that of the TPO‑handled group. Moreover, <em>in vitro</em> experiments indicated that therapy with 100 <em>µ</em>g/ml ASPS exhibited the utmost impact on colony formation.
ASPS attenuated cell apoptosis in megakaryocytic cells through inhibiting the mitochondrial <em>caspase</em>‑Three signaling pathway. In conclusion, ASPS promoted hematopoiesis in irradiated myelosuppressive mice probably through enhancing hematopoietic stem/progenitor cell proliferation and inhibiting megakaryocytes apoptosis.
Anti-Inflammatory, Barrier-Protective, and Antiwrinkle Properties of Agastache rugosa Kuntze in Human Epidermal Keratinocytes
Tripartite motif‑containing <em>14</em> regulates cell proliferation and apoptosis in cervical most cancers through the Akt signaling pathway
Tripartite motif‑containing (TRIM) <em>14</em> is a protein of the TRIM household. Research have indicated that TRIM<em>14</em> could also be used as an oncogene in tumor cells, comparable to osteosarcoma, non‑small cell lung most cancers and breast most cancers by totally different pathways. Nevertheless, the capabilities of TRIM<em>14</em> in cervical most cancers cells stay unclear. Due to this fact, this examine aimed to research the capabilities of TRIM<em>14</em> in cervical most cancers cells and its underlying mechanism. Caski cells stably expressing TRIM<em>14</em> and SiHa, and HeLa cells stably expressing TRIM<em>14</em> brief hairpin RNA have been constructed by lentivirus‑mediated overexpression or knockdown programs.
The consequences of TRIM<em>14</em> on proliferation and apoptosis of cervical most cancers cells have been detected by Cell Counting Equipment‑8 (CCK‑8) assay and stream cytometry, respectively. As well as, reverse transcription‑quantitative (RT‑q) PCR and western blotting have been used to research the expression ranges of TRIM<em>14</em> and of signaling pathway marker protein together with P21, <em>caspase</em>‑3, cleaved <em>caspase</em>‑3, Akt and phosphorylated Akt. The outcomes of RT‑qPCR and western blotting revealed that TRIM<em>14</em> was extremely expressed in human cervical most cancers tissues and cell traces in contrast with adjoining regular tissues and regular cervical epithelial cells. TRIM<em>14</em> additionally regulated cell proliferation and apoptosis of human SiHa, HeLa and Caski cervical most cancers cell traces by the Akt signaling pathway. Moreover, TRIM<em>14</em> protein ranges have been associated to the scientific and pathological options of cervical most cancers.
 Caspase 8 Blocking Peptide |
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33R-10438 |
Fitzgerald |
50 ug |
EUR 349 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Caspase 8 antibody, catalog no. 20R-1428 |
Caspase 8 Blocking Peptide |
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33R-10456 |
Fitzgerald |
50 ug |
EUR 349 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Caspase 8 antibody, catalog no. 20R-1477 |
Caspase 8 Blocking Peptide |
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33R-10464 |
Fitzgerald |
50 ug |
EUR 349 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Caspase 8 antibody, catalog no. 20R-1508 |
Caspase 8 Blocking Peptide |
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33R-10594 |
Fitzgerald |
50 ug |
EUR 191 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Caspase 8 antibody, catalog no. 70R-11562 |
Caspase 8 Blocking Peptide |
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33R-10652 |
Fitzgerald |
50 ug |
EUR 191 |
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Caspase 8 antibody, catalog no. 70R-11657 |
 Caspase-8 Blocking Peptide |
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3259BP-50 |
Biovision |
|
EUR 153 |
Caspase 8 Blocking Peptide |
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20-abx062348 |
Abbexa |
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- Shipped within 5-10 working days.
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Caspase 8 Blocking Peptide |
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AF6442-BP |
Affbiotech |
1mg |
EUR 195 |
 Active Caspase 8 Blocking Peptide |
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33R-10690 |
Fitzgerald |
50 ug |
EUR 191 |
|
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of Active Caspase 8 antibody, catalog no. 70R-11724 |
 Caspase 8 p18 Blocking Peptide |
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20-abx162092 |
Abbexa |
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- Shipped within 5-10 working days.
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 Blocking Peptide) Caspase 8 (pS347) Blocking Peptide |
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20-abx062346 |
Abbexa |
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- Shipped within 5-10 working days.
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 Blocking Peptide) Caspase 8 (pY380) Blocking Peptide |
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20-abx062347 |
Abbexa |
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- Shipped within 5-10 working days.
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 Blocking Peptide) Phospho-Caspase 8 (Ser347) Blocking Peptide |
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AF3442-BP |
Affbiotech |
1mg |
EUR 195 |
 Blocking Peptide) Caspase 8 (Cleaved-Asp384) Blocking Peptide |
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AF5267-BP |
Affbiotech |
1mg |
EUR 195 |
 Blocking Peptide) Phospho-Caspase 8 (Tyr448) Blocking Peptide |
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AF8103-BP |
Affbiotech |
1mg |
EUR 195 |
 1730 8 SNAP-SEAL 8 OZ |
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1730-8 |
CORNING |
100/pk |
EUR 74 |
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Description: Disposable Plastic; Plastic Containers |
 Individual Reaction Mix 8 |
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G065-8 |
ABM |
200 reactions |
EUR 167 |
 Caspase-8 Antibody |
|
3020-100 |
Biovision |
|
EUR 349 |
Caspase-8 Antibody |
|
3020-30T |
Biovision |
|
EUR 146 |
 Caspase 8 protein |
|
30R-2324 |
Fitzgerald |
25 units |
EUR 352 |
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Description: Purified recombinant Human Caspase 8 protein |
Caspase 8 protein |
|
30R-2328 |
Fitzgerald |
25 units |
EUR 281 |
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Description: Purified recombinant Mouse Caspase 8 protein |
 caspase-8 antibody |
|
22977-100ul |
SAB |
100ul |
EUR 390 |
Caspase-8 Antibody |
|
24296-100ul |
SAB |
100ul |
EUR 390 |
Caspase-8 Antibody |
|
24297-100ul |
SAB |
100ul |
EUR 390 |
 Caspase 8 antibody |
|
20R-1428 |
Fitzgerald |
100 ug |
EUR 673 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
20R-1477 |
Fitzgerald |
100 ug |
EUR 673 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
20R-1508 |
Fitzgerald |
100 ug |
EUR 673 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
20R-2496 |
Fitzgerald |
50 ug |
EUR 281 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase-8 Antibody |
|
3158-100 |
Biovision |
|
EUR 316 |
Caspase-8 Antibody |
|
3158-30T |
Biovision |
|
EUR 146 |
Caspase 8 antibody |
|
70R-11562 |
Fitzgerald |
100 ug |
EUR 417 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
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70R-11657 |
Fitzgerald |
100 ug |
EUR 403 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
70R-12555 |
Fitzgerald |
100 ul |
EUR 457 |
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Description: Affinity purified Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
70R-30657 |
Fitzgerald |
100 ug |
EUR 327 |
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Description: Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
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70R-13850 |
Fitzgerald |
100 ug |
EUR 322 |
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Description: Affinity purified Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
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70R-14063 |
Fitzgerald |
100 ug |
EUR 322 |
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Description: Affinity purified Rabbit polyclonal Caspase 8 antibody |
Caspase 8 antibody |
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70R-14272 |
Fitzgerald |
100 ug |
EUR 322 |
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Description: Affinity purified Rabbit polyclonal Caspase 8 antibody |
 caspase 8 Antibody |
|
39265-100ul |
SAB |
100ul |
EUR 390 |
Caspase 8 antibody |
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10R-6739 |
Fitzgerald |
100 ug |
EUR 781 |
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Description: Mouse monoclonal Caspase 8 antibody |
Caspase-8 Antibody |
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48679-100ul |
SAB |
100ul |
EUR 333 |
Caspase-8 Antibody |
|
48679-50ul |
SAB |
50ul |
EUR 239 |
Caspase-8 Antibody |
|
49184-100ul |
SAB |
100ul |
EUR 333 |
Caspase-8 Antibody |
|
49184-50ul |
SAB |
50ul |
EUR 239 |
Caspase 8 antibody |
|
70R-49471 |
Fitzgerald |
100 ul |
EUR 287 |
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Description: Purified Polyclonal Caspase 8 antibody |
Caspase 8 antibody |
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70R-49472 |
Fitzgerald |
100 ul |
EUR 287 |
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Description: Purified Polyclonal Caspase 8 antibody |
Caspase 8 antibody |
|
70R-49473 |
Fitzgerald |
100 ul |
EUR 244 |
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Description: Purified Polyclonal Caspase 8 antibody |
 Caspase 8 Antibody |
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AF6442 |
Affbiotech |
200ul |
EUR 304 |
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Description: Caspase 8 Antibody detects endogenous levels of total Caspase 8. |
 Human IL-8 Recombinant Protein |
|
R00423-8 |
BosterBio |
5ug/vial |
EUR 259 |
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Description: Interleukin-8 (IL-8), also known as CXCL8, is an ELR-positive CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. Human IL-8 Recombinant Protein is purified interleukin-8 produced in yeast. |
 Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 |
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M00334-8 |
BosterBio |
100ul |
EUR 375 |
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Description: Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 tested in WB, IHC, reactive to Human |
 Caspase Family Inhibitor Set III |
|
K210-8-25 |
Biovision |
|
EUR 403 |
 Caspase 8 Polyclonal Antibody |
|
27230-100ul |
SAB |
100ul |
EUR 252 |
Caspase 8 Polyclonal Antibody |
|
27230-50ul |
SAB |
50ul |
EUR 187 |
Caspase 8 Polyclonal Antibody |
|
27526-100ul |
SAB |
100ul |
EUR 252 |
Caspase 8 Polyclonal Antibody |
|
27526-50ul |
SAB |
50ul |
EUR 187 |
 Active Caspase 8 antibody |
|
70R-11724 |
Fitzgerald |
100 ug |
EUR 436 |
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Description: Rabbit polyclonal Active Caspase 8 antibody |
) Caspase 8 antibody (Ser347) |
|
70R-30656 |
Fitzgerald |
100 ug |
EUR 327 |
|
Description: Rabbit polyclonal Caspase 8 antibody (Ser347) |
 Caspase-8 Rabbit pAb |
|
A11324-100ul |
Abclonal |
100 ul |
EUR 308 |
Caspase-8 Rabbit pAb |
|
A11324-200ul |
Abclonal |
200 ul |
EUR 459 |
Caspase-8 Rabbit pAb |
|
A11324-20ul |
Abclonal |
20 ul |
EUR 183 |
Caspase-8 Rabbit pAb |
|
A11324-50ul |
Abclonal |
50 ul |
EUR 223 |
Caspase-8 Rabbit pAb |
|
A11450-100ul |
Abclonal |
100 ul |
EUR 308 |
Caspase-8 Rabbit pAb |
|
A11450-200ul |
Abclonal |
200 ul |
EUR 459 |
Caspase-8 Rabbit pAb |
|
A11450-20ul |
Abclonal |
20 ul |
EUR 183 |
Caspase-8 Rabbit pAb |
|
A11450-50ul |
Abclonal |
50 ul |
EUR 223 |
 Antibody) Caspase-8 (Active) Antibody |
|
3259-100 |
Biovision |
|
EUR 338 |
Caspase-8 (Active) Antibody |
|
3259-30T |
Biovision |
|
EUR 146 |
 Caspase-8, human recombinant |
|
1088-100 |
Biovision |
|
EUR 452 |
Caspase-8, human recombinant |
|
1088-25 |
Biovision |
|
EUR 283 |
Active Caspase 8 antibody |
|
10R-8030 |
Fitzgerald |
100 ug |
EUR 558 |
|
Description: Mouse monoclonal Active Caspase 8 antibody |
 Caspase-8, mouse recombinant |
|
1188-100 |
Biovision |
|
EUR 359 |
Caspase-8, mouse recombinant |
|
1188-25 |
Biovision |
|
EUR 207 |
 Caspase-8 Polyclonal Antibody |
|
42620-100ul |
SAB |
100ul |
EUR 333 |
 Caspase-8 Monoclonal Antibody |
|
40483-100ul |
SAB |
100ul |
EUR 252 |
Caspase-8 Monoclonal Antibody |
|
40483-50ul |
SAB |
50ul |
EUR 187 |
Caspase-8 Polyclonal Antibody |
|
40674-100ul |
SAB |
100ul |
EUR 252 |
Caspase-8 Polyclonal Antibody |
|
40674-50ul |
SAB |
50ul |
EUR 187 |
) Human Caspase-8 (CASP8) |
|
1-CSB-RP181094h(A3) |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
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100ug
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10ug
-
1MG
-
200ug
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500ug
-
50ug
|
- MW: 21.9 kDa
- Buffer composition: Tris-based buffer with 50% glycerol.
|
|
Description: Recombinant Human Caspase-8(CASP8),partial expressed in E.coli |
Caspase-8 Rabbit pAb |
|
A0215-100ul |
Abclonal |
100 ul |
EUR 308 |
Caspase-8 Rabbit pAb |
|
A0215-200ul |
Abclonal |
200 ul |
EUR 459 |
Caspase-8 Rabbit pAb |
|
A0215-20ul |
Abclonal |
20 ul |
EUR 183 |
Caspase-8 Rabbit pAb |
|
A0215-50ul |
Abclonal |
50 ul |
EUR 223 |
) Caspase 8 antibody (Tyr380) |
|
70R-32592 |
Fitzgerald |
100 ug |
EUR 327 |
|
Description: Rabbit polyclonal Caspase 8 antibody (Tyr380) |
 Antibody) Caspase 8 (CASP8) Antibody |
|
20-abx009497 |
Abbexa |
-
EUR 300.00
-
EUR 439.00
-
EUR 189.00
|
|
- Shipped within 5-10 working days.
|
 Antibody) Caspase 8 (pT380) Antibody |
|
20-abx009708 |
Abbexa |
-
EUR 314.00
-
EUR 467.00
-
EUR 203.00
|
|
- Shipped within 5-10 working days.
|
 Antibody) Caspase 8 (pS347) Antibody |
|
20-abx009709 |
Abbexa |
-
EUR 314.00
-
EUR 467.00
-
EUR 203.00
|
|
- Shipped within 5-10 working days.
|
Caspase 8 (CASP8) Antibody |
|
20-abx005603 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
- Shipped within 5-10 working days.
|
Caspase 8 (CASP8) Antibody |
|
20-abx100466 |
Abbexa |
-
EUR 286.00
-
EUR 133.00
-
EUR 690.00
-
EUR 356.00
-
EUR 244.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-7 working days.
|
Caspase 8 (CASP8) Antibody |
|
20-abx100467 |
Abbexa |
-
EUR 356.00
-
EUR 133.00
-
EUR 996.00
-
EUR 495.00
-
EUR 300.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-7 working days.
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Caspase 8 (CASP8) Antibody |
|
20-abx100468 |
Abbexa |
-
EUR 356.00
-
EUR 133.00
-
EUR 1024.00
-
EUR 509.00
-
EUR 300.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-7 working days.
|
Caspase 8 (CASP8) Antibody |
|
20-abx100469 |
Abbexa |
-
EUR 411.00
-
EUR 133.00
-
EUR 1135.00
-
EUR 551.00
-
EUR 314.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
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- Shipped within 5-7 working days.
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Caspase 8 (CASP8) Antibody |
|
20-abx100470 |
Abbexa |
-
EUR 453.00
-
EUR 133.00
-
EUR 1288.00
-
EUR 620.00
-
EUR 342.00
|
-
100 ug
-
10 ug
-
1 mg
-
200 ug
-
50 ug
|
- Shipped within 5-12 working days.
|
Caspase 8 (CASP8) Antibody |
|
20-abx111426 |
Abbexa |
|
|
- Shipped within 5-10 working days.
|
CCK‑Eight assay and stream cytometry demonstrated that TRIM<em>14</em> expression might promote cervical most cancers cell proliferation and autophagy suppression. Taken collectively, TRIM<em>14</em>‑induced cell proliferation and apoptosis inhibition might by evoked by the activation of the Akt pathway. This examine demonstrated the position of TRIM<em>14</em> in cervical most cancers, and divulges its mechanism of motion as a possible therapeutic goal for cervical most cancers.
 ELISA Kit)
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